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- Posts: 16
- Joined: Wed May 25, 2022 2:42 pm
Can I still perform my measurements at 190 nm? I also use water as a mobile phase. Doesn't this take water off as a blank anyway?
Thank you!
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
TylerSmith123 wrote:
Getting an accurate signal of any compound at the wavelength 190 nm will be hard due to, as you noted, the absorption coming from your mobile phase (and anything else that may be in there, additives, buffers). ...
As for your comment about "taking the water off as a blank", I'm not sure exactly what this means? If you're assuming that when you inject a blank of water that the system will subtract that blank from your future analysis then that is not true.
clinephd wrote:
Can I still perform my measurements at 190 nm?
Chromavore wrote:
If you could share more of your method parameters that could be useful,
Consumer Products Guy wrote:
I rarely used low wavelengths for anything. But we had RID, ELSD, conductivity detectors too.
DR wrote:Consumer Products Guy wrote:
I rarely used low wavelengths for anything. But we had RID, ELSD, conductivity detectors too.
^those detection modes are all more appropriate for sugars than UV.
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