-
- Posts: 16
- Joined: Wed May 25, 2022 2:42 pm
I am having some problems with my RI detector. I do not perform standard HPLC analyses with my HPLC system, but do adsorption experiments with it. For this, I pack an HPLC empty column with adsorbent powder, inject my adsorptive solutions and then want to determine isotherms based on the resulting chromatogram. I used ultrapure water as the eluent. My samples are various sugars that were dissolved in ultrapure water.
My big problem now is that two peaks overlap in my chromatogram: the sugar peak and another peak. Since the eluent and the injection solvent are identical, I strongly suspect that the second peak is caused by dissolved gases in my sample.
Normally, such problems can be solved by the column packing or column length. But I don't have too much room for manoeuvre, because pressure problems arise if the columns are too long. (As I said, these are self-packed columns, which in any case cannot be packed perfectly homogeneously).
Does anyone have an idea how else I could get around/fix/solve this peak overlay? I am very grateful for any help!