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- Posts: 405
- Joined: Wed Jul 18, 2012 6:45 am
- Location: Germany
I have trouble with an isocratic method (C18 column, 10 % ACN, 90 % Tetrabutylammonium hydrogen sulfate/phosphate), and in some (by far not all) injections I see massive interferences:
"normal" chromatogram:
I have cleaned all solvent lines with water, nitric acid, water, isopropanol, Acetone/acetonitrile and back to water. I have cleaned the syringe, changed the pre-column and flushed the UV flow cell.
Any other idea?
Thanks a lot
Jörg