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- Posts: 6
- Joined: Thu Jan 31, 2019 8:36 pm
Column: Phenomonex NH2 4.6x150mm, 5um P/N 00F-4378-E0
Mobile phase: Phosphate Buffer at pH 7.5 with Acetonitrile mixture(250/750 mL).
Wavelength: 195 nm.
My problem is the baseline drift. Normally, it takes me to do a few sample injections to achieve a good baseline. I don't understand why Buffer solution causes the baseline drift. I run a different UV method which use Phosphate Buffer to test Thiamine with C18 column at 254 nm. I have no baseline drift. Can anyone explain why the baseline-drifting only happens in some circumstance? Is there any good paper about baseline-drifting? Thanks