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- Posts: 1
- Joined: Sun Apr 21, 2019 6:23 pm
I am currently trying to seperate compound from an active fraction of a sea star. Based on the nmr i ran earlier, there are sugar and nucleoside compounds in it. I had tried various methods, and this is the method with the best seperation:
This is the link to the chromatogram:
https://drive.google.com/file/d/1jaWg6J ... p=drivesdk
Solvent A: 0.1% TFA in H20
Solvent B: pure Acetonitrile
Flow rate: 1.0 ml/min
Temperature: Ambient
Tyoe of column: Purospher Hibar C18 encapped( 5 micrometer)
Method: 0 to 10th minute: Solvent B from 0% to 25%
10th to 15th minute: Solvent B from 25% to
50%
Can anyone please suggest methods to improve this chromatogram?
I have tried reducing the flow rate to 0.5ml/min and tweaking the gradient, the peaks became wide and separation is not good.
Thabk you in advance.