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- Posts: 405
- Joined: Wed Jul 18, 2012 6:45 am
- Location: Germany
I have an annoying issue on my Ultimate 3000 binary pump system: There is periodic ripple in the UV chromatogram, but I'm not sure if it is related to pressure or not. The pressure also has spikes, but these are rather small and look OK to me.
Method details:
A = H2O + 0.1 % TFA
B = ACN + 0.085 % TFA
Gradient from 10 -> 25 % B over 10 min, wash step to 90 % and followed by re-equilibration.
I have read in another thread that lamp could be a problem with TFA at 220 nm. The lamp is close to 2000 h old, but passed the UV linearity and nois/drift test in the instrument PQ last week.
Thanks for any ideas,
Jörg