Tributyrin

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Any experience in getting Tributyrin (polar TG) to stick to an LC column.
Using ELSD-MS compatible solvents (MeOH, ACN, Acetone), have been
unsuccessful in getting these low MW Triglycerides to stick to C18 columns.

Thanks for your help

It won't be retained if you use those solvents straight. You'll need to dilute with water or dilute aqueous acetic or formic acid depending upon what else might be in your sample. I'd suggest increasing the aqueous content of your MP in 5 to 10 percent increments and injecting until the tributyrin is where you'd like it. Alternately, you can run a gradient from (I'd guess) about 30% organic to 95% organic over 20 minutes or so and see where you land.

Your ELSD should be able to handle water easily enough, though the more you have, the lower your sensitivity will be. You'll likely need to fine tune your pressure and temperature settings and you should be fine.

PS...In my experience at least, acetone is a good solvent to use for enhancement of ELSD sensitivity, just be sure to buy the cleanest grade acetone you can possibly get. If you get the selectivity you need and you're not using a UV detector in front of your ELSD, I'd recommend using acetone as the organic portion of your MP.
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry