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- Posts: 13
- Joined: Thu Mar 08, 2012 11:59 pm
We are using 10mL headspace vials with gray butyl septa (boiled prior to use to clean and prevent leaking) and capped with aluminum crimps to store 12mL atmospheric gas samples. We will be measuring CH4, N2O, and CO2 on a GC with an FID, ECD, and TCD, respectively. Our GC carrier is He.
As of now (pre-experiment trials), we've been evacuating the vials prior to sampling. Unsurprisingly, even in new vials, our He "blanks" still have detectable N2 and CO2. If we reuse vials that have acetylene in them previously (part of our sample matrix) by reevacuating them, the C2H2 peak shows up in He "blanks".
So, what is the best way to prepare my headspace vials to avoid contamination from the air and how do I clean my vials between uses? Is there a recommended method for prepping vials? Should they be washed between uses? Because I'm already dealing with trace gases, I need to avoid diluting my samples (i.e., injecting 12mL of sample into a vial flushed with 10mL of inert gas (He, N) is not an option).
Thanks in advance,
GHG Girl