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Selected Ion Monitoring (SIM) mode

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

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Selected Ion Monitoring (SIM) mode

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okarvi
Hi there,
Sorry for asking this very basic question. I want to determine the molecular weight of a peptide (20 AAs), using Agilent LC/MS. Can I use SIM mode to determine the exact MW of my peptide. Sometimes, I am not able to get exact mass using scan mode, but with SIM mode, it mostly gives the correct mass. Is SIM mode acceptable for publication.
Thank you all for your response.

Re: Selected Ion Monitoring (SIM) mode

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lmh
what instrument are you using? "Exact mass" usually implies a ToF or similar, but SIM/scan usually implies a quadrupole...
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