by
DSP007 » Sun Apr 17, 2011 7:55 pm
Example
http://www.icct.ru/Practicality/Papers/ ... 03-Big.jpg- MiLiChrome-1 (model1968-1983 year, apparently the world's first UPLC (column 2x64 mm) . Architecture is very open - to the modern computer can be connected through the staff connector) . Pumps (syringe 2.5 ml , 5MPa 0.003-1ml/min) - glass, tantalum, Teflon, Teflon-communication, cell - quartz, Teflon (holder - steel), column- stainless steel or glass, silicagel or C18RP-silicagel (Lachema ChSFR). The concept of exclusion sorbent 40 years ago was not known, although (but) the "KCK" ( silicagel for draining gas, pores not normalized 300-1000 A) is normal exclusion sorbent.
Reodain Injector ? Injector was not there in principle, the direct input of sample into the column. Gradient pump? Too too much - gradient (steps) was created directly in the syringe.
However installing in the series since 1984 MiLiChrome -2 - was much steeper. This was drived by computer at KR580VM (analog i8080), haved autosampler in a minimal configuration, and allowsed the installation of the second pump for the linear gradient. This form
http://milihrom.narod.ru/pic1.jpgmodifed up to the current 6 modification. An iron "coffin" is replaced by a plastic casing, IRPS (RS-232)-USB ,i-8080- Pentium Dual core and add electronic thermostat.