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- Posts: 3
- Joined: Mon Mar 21, 2016 6:21 pm
The calculation I am using for the spike is:
%R = ((Spiked sample result - unspiked sample result)*100%)/ (known spike added concentration * DF)
DF = Sample volume/total volume of sample plus spike
When I made my standards for 2 g/l I added 200 microL to 800 microL of water. (10 g/l stock solution).
For the spike I added 200 microL to 800 microL of beer (beer is sample matrix)
Doing the spike this way is there a dilution factor that comes into play?
The actual data I am using is as follows:
Spiked sample - 2.15
Unspiked - 0
Expected Spike - 2.8735