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- Posts: 28
- Joined: Fri Mar 11, 2016 3:41 pm
What do you guys think?
To not start another thread:
How important is the observation of a qualifier ion in addition to a quantifier ion in target analysis?
Best regards
Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.
MrGC wrote:
To not start another thread:
How important is the observation of a qualifier ion in addition to a quantifier ion in target analysis?
James_Ball wrote:
Sometimes you just can't find an isotopically labeled version of the analyte of interest and it would be very cost prohibitive to have one custom made, therefore another functionally similar analyte that you do not expect to find in the sample matrix will work, and the closer it is in retention time to the analyte of interest the better, for evaluating matrix effects in the MS source.
Qualifiers are not always required but the more you have, the higher confidence you can have that you do not have a false positive result.
Kenn wrote:
Some of our European clients are now asking for quantitation to be performed and reported for both the primary and secondary mass transitions in method validations. A new SANCO guideline that I believe they are misinterpreting but what are you to do, they are the clients.
Rndirk wrote:
So you have to set up your processing method to calibrate and calculate on ion 1, and again on ion 2 giving you twice the amount of data/QC/...
This is where I would personally draw the line
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