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- Posts: 12
- Joined: Thu Dec 14, 2017 1:35 pm
Here is my question. When you do the compound tuning as well as FIA, has the solvent used dissolving your target compounds to be the same as your mobile phase? If yes, why and how would this affect my later method development?
At the very beginning, I used isopropanol to dissolve my standard because I will use it as my mobile phase for chiral separation using a chiral column. But it turns out that the separation is not good. And I decided to separate the achiral analytes first using a C18 column (MP: ACN) by using the same values of DP, EP, etc. tuned at the beginning. And later I found my repeatability is so poor with intensities jumping up and down without any rules. So now I am doing the compound tuning and FIA again using the same solvent as my MP.
Thanks for your attention.
Best regards,
Kenny