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- Posts: 4
- Joined: Sat Sep 30, 2017 7:27 am
I need to buffer a urine sample at pH7 for a glucuronide hydrolysis step. What would you suggest in terms of buffers that would be compatible with ESI-LCMS? pKa of Formic and Acetic acid are both a bit too low. It's a dilute and shoot or straight protein precipitation method, no SPE or further sample clean-up. I've heard of bicarbonate buffers being used, would these be stable at high temperatures (50deg) in a water bath?
Thanks for your assistance!