Baseline trouble shooting

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

5 posts Page 1 of 1
I have a problem with LC-MS/MS baseline. It is spiky with equal frequency. I am not sure how to explain and I do not know how to attach image to this post. Please help.

The instrument pressure is normal based on online calculator.
Gift wrote:
I have a problem with LC-MS/MS baseline. It is spiky with equal frequency. I am not sure how to explain and I do not know how to attach image to this post. Please help.

The instrument pressure is normal based on online calculator.

Here you are:
viewtopic.php?f=1&t=2617
Thank you for the tip.

Here is the TIC I was talking about. Blue line is the TIC, red is pressure.
Image

When I changed the dwell time the frequency of the spikes changes too.
Tune situation? Could you attache an screenshot of your last tune?
what are you doing? If this is a mass spec method that is carrying out multiple different scans (for example, an MRM method, or a method collecting full scan data and MS2 simultaneously) then a lot of systems will display a total ion chromatogram that is the sum of everything going on, which will naturally zig-zag up and down as the instrument measures first a full scan, or an intense ion, and then an MS2 or weaker ion. When you view the data in software that filters out the appropriate data, they may all turn into nice sensible baselines.
5 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry