pH, ion mode and acid/basic analytes
Posted: Thu Sep 09, 2004 2:41 pm
I´ve learned that I should use high pH for acids to produce ions in negative mode and vice versa (correct?), but then I read that an adduct formed from formic acid is
[M-H+HCO2H]-. And I, myself, have experience of acid analytes that form [M-H]- in ES- with formic acid. Would the response be better in ES+ or with basic pH?
Can anyone explain a little bit more about this (pH, ion mode and acid or basic analyte)?
[M-H+HCO2H]-. And I, myself, have experience of acid analytes that form [M-H]- in ES- with formic acid. Would the response be better in ES+ or with basic pH?
Can anyone explain a little bit more about this (pH, ion mode and acid or basic analyte)?