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- Posts: 33
- Joined: Wed Dec 08, 2004 4:16 am
I am recently reading about the journal paper applying isotope dilution technique (IDT) in pesticide residue analysis with LCMSMS.
From what am I understand, the use of IDT help to compensate or correct the error that occurred during extraction (assuming internal standard (IS) was added prior to sample extraction) and matrix effect.
However, I noted that several journal authors mentioned that they also carried out recovery study for the method they have developed. The recovery study was carried out by comparing the area ratio of analyte and IS for sample spiked before extraction to the same ratio for analyte and IS added to the extracted solution (analyte and IS added after extraction).
My confuse is if IDT corrected the error or problem with recovery, why did the authors still need to carry out recovery study? What actually the reason to conduct such study and does it mean if the recoveries are bad, the method developed is also bad?
Hope to get explanations from peoples here who has experiences in using IDT.
Thank you.