Matrix effect

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

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Hello,
Would be great if someone could give me a hint what can cause a vary between matrix effects on quantitative and qualitative mass transition in
LC-ESI-MS/MS. The precursor ion is the same for both transitions.
Different is bigger than 100%
If i understand your post correctly, what you're seeing is not the matrix effect in LC-ESI-MS.

While the matrix effect is a very complex process and not (yet) understood on a molecular level, there is a consensus that suppression/enhancement happens mostly during the process of going from ions in liquid to ions in the gas phase in ESI-MS. In other words, it happens before the collision process.

You see a difference in fragmentation pattern of a single precursor ion to 2 daughter ions in sample, relatively to the same precursor in solvent, right? Are you looking at a spiked or an unknown sample?
2 posts Page 1 of 1

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