-
- Posts: 3
- Joined: Fri Mar 23, 2018 10:06 pm
First of all, this is my first time here and nice to meet you all.
I am currently working on my research project using QTOF. Where I need to find the stability of synthetic Cannabinoids. Since synthetic cannabinoids are very expensive I am optimizing this method using benzocaine which has similar properties.
I have been using a 0.1% formic acid in water and 0.1% formic acid in acetonitrile(B) in positive mode. and the column I used was C18 column
The gradient I used was as follows with 2microliter injection of 10ppm benzocaine,
B increased until 0-100% over 10 mins time followed by a equilibrium step of 1 min, after that over another 1 minute B goes to the initial 0%.
This was done in positive mode and the mass spectrum was extracted at 166.
So far I haven't been able to witness peaks for benzocaine and I have been seeing other peaks interfering even in the BPI chromatogram. Any idea what I am doing wrong.
Thank you.