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Using in-house methodology for HPLC UV-Vis, we don't see this matrix effect, so it is specifically related to GC-FID methodology.
I am looking to analyze Prallethrin in a liquid formula that contains some other compounds and a solvent system that is propylene carbonate, peg 400 and pluronic.
I guess my main question is what types of phonemena may be causing me to see a reduced area in both the active peak of interest as well as the internal standard (although the decrease in ISTD area is not nearly as great as the active of interest)? Especially phenomena that would be specific to GC-FID that I do not observe in HPLC UV-Vis?
My overall experience with analyzing more complex samples on GC-FID is significantly less than HPLC, so I'm not necessarily sure what I should look at first.
I have great resolution between Prallethrin and the ISTD (Triphenyl Phosphate), and all of the other components in the formulation elute well before the peaks of interest as well. I can share a chromatogram if necessary but it doesn't look like I'm seeing a lower area because of an issue with co-eluting peaks or a discrepancy between integrating nearby peaks properly.
Sorry this was a bit long-winded.