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- Posts: 1
- Joined: Tue Feb 27, 2018 2:31 pm
I am analyzing a reaction mixture by GC and I am facing trouble quantifying the desired product.
The technical department has gone through the process of calibration curve so, I have what They call a response factor (KF=0,63) between the standard and the product of interest. They determined this factor with a mass of standard = 18,90 mg and a mass of clean product = 19,3 mg.
After performing my reaction, I prepare a sample for GC analysis. To do that, I add in a vial a random weighted amount (mg) of the reaction mixture, the standard and the solvent. Then I submit the sample for GC.
I received a spectrum where there are different peaks. As I know the retention time of the product and the standard, it is easy to identify them. I use the following formula to calculate the amount of product I am having from the reaction: m(product)= Area(product)/Area (standard) * KF * m (standard)
Question: Calulating the mass following the above steps never give me the right amount of product I have (I have perform a purification of the reaction mixture to know how much I have and see if it was accurate). Is there any steps missing in my calculation?
Question: Also, as I have prepared a sample for GC (dilution of the reaction mixture), how does it represent the reaction mixture? I never used the dilution I have made for the calculation of the mass of the product. If I have to, how should I do that?
Thank you in advance for your help.