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- Posts: 4
- Joined: Wed Sep 20, 2017 12:09 pm
Now im working on determination of acrylamide in food by GC (ECD). But i have some problems.
Firstly its calibration curve.
I have 6 standard solutions.
Preparation of these solutions: I addKBr, hydrobromic acid ( to pH 1-3), bromine water in water solution of AA
Then bromination during 1 hour in refrig. After i need neutralize the bromine with 1M sodium thiosulfate.
Then I add sodium sulfate and stir, then extraction the aqueous solution twice with 10 ml of ethyl acetate 10 min each extraction.Add triethylamine. Dry organic phase with Na2SO4. Evap to dryness. Add to it 2 ml of ethylacetate and GC.
Problem: calibration curve is not stable. Why?
And another question: i must use salt (Na2SO4) before extraction with ethylacetate?
Thx a lot!