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- Posts: 4
- Joined: Mon Jan 29, 2018 1:26 pm
I’m going to start new research which the samples are from mice tissue like liver, kidney, spleen, skin, bone, muscle.
Is anybody out there have experience extracting the protein from those kind of sample ? I’m quite new in this merit. My laboratory is provided with ultrasound sonicator. I plan to use him, but I’m stuck with the insoluble phase. After sonicated, I filter the supernatant (yielded from centrifugation 10000g 5min) with milipore filter, but result came out with the insoluble material in it. I need to add the Nitric acid to the supernatant and it getting turbid.
I’m not sure what to do. All I do is following what my seniors did and that is the result.
So, here I am come for a help.