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- Posts: 1
- Joined: Thu Oct 19, 2017 5:21 pm
I'm quite new to analytical chemistry, so I've maybe got a noob question for you:
I have do develop a method to measure polyamines in wheat samples by HPLC with UV detection (254 nm), pre-column derivatization with dansylchloride. I can already measure and quantify desired polyamines (less than compared with HPLC-MS, but that's another question...). Most protocols recommend filtration with 0.45µm filters, and a very few centrifugation, some both.
So, my simple question is:
Can i centrifuge my samples (10000g or more, 5 minutes) prior injecting into my HPLC system, instead of filter them with 0.45 filters? Or could this eventually harm my column, or even lose some of my analytes, other disadvantages? It would be much easier, less time and money consuming to simply centrifuge them.