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- Posts: 2
- Joined: Mon Mar 12, 2018 10:39 am
I am developing a method using an ion pair on a C18 column.
The mobile phase: phosphate buffer 50mM pH 3.5 + Sodium dodecansulfate and CH3CN (850: 15).
I get a good retention of the peak, but it is split and asymmetric.
I read that this could be due to:
" if the rate of interconversion between the free and paired analyte is slow compared to the chromatographic retention time scale".
How can I solve it?
Thank you.
Gianna