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- Posts: 46
- Joined: Fri May 18, 2007 11:48 pm
But, ironically, my question actually has to do with a case where you can't do modeling. So let me explain. We are currently looking at a situation where we can't use modeling, because there are too many small peaks, such that we can't track them (if we were setup on an LC/MS then it might be doable, but we just have the UV). What we want to evaluate is combinations of Methanol and ACN in mobile phase B. So my question is: Do you think it is reasonable to evaluate:
first: 25/75 ACN/MeOH
then: 75/25 ACN/MeOH
And to continue narrowing it down from there. Or is there a chance that 100% MeOH or 100% ACN (neither of which would be run, by this approach) might give something quite different, which we wouldn't know about unless we actually ran them.
Thanks for any suggestions.