-
- Posts: 1
- Joined: Thu Mar 01, 2018 9:58 pm
I am experiencing some high pressure issues with our Agilent 1100 hplc. Normal equilibration pressures at 1.5 ml/min are consistent at around 120 bar. Now, equilibration pressure at 1.5 ml/min are +200 bar.
What I have tried:
Removed column, flow to waste, low- no pressure observed.
Flushed column forward and reverse w different solvents. No relief of high pressure.
New mobile phases, completely flushed system. No relief of high pressure.
Installed a brand new column, observed same high pressure.
I am having trouble locating the problem and think it may be in the pump. Any help is appreciated from the community. Thank you all.