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- Posts: 28
- Joined: Mon Feb 18, 2013 4:44 am
But I think we all know, as well, that there are any number of 'Extra-column Factors' that can prevent one from obtaining the full efficiency (or 'peak sharpness') that we should get in theory.
So my interest here is to take a poll, asking the basic question: What extent of the full benefit have people generally observed? My sense is that when we go below 3 um we will typically not see the full, theoretical, benefit. In fact, my sense is that it may usually be closer to 50% of this. I have even had one or two cases where I went from 5 um to 3 um (to better separate some closely resolved peak), and in fact, the peaks were not any sharper with 3 um column (which, of course, they should be from theory).
Now one last thing: We are very interested to hear everyone's feedback. But, when responding, please steer clear of "Explanatory Answers" such as explaining how the connecting tubing should not be too long and should have a narrow ID, and the injection volume.......etc etc......I think most people know these things by now. What we are looking for is people's real world observations....."I went to a smaller diameter particle and......I got all the expected benefit......or none of it......or half of it......".
Thanks very much in advance.