by
kuikz » Fri Feb 02, 2018 9:02 pm
Hi, maybe someone can tell me how this is possible...a friend in an other company is developing a UPLC method for an API. I know she works with Waters Aquity Systems with a PDA as detector, and today came a weird question. She asked how it is posible to have different absorption values but still the same concentration?
She says the concentrations are correct and there is no significant RT or lambda max shifting. Peak symmetry is constant. I dont know if this is enough information but i dont have more, maybe some one can explain it?