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- Posts: 104
- Joined: Tue Aug 04, 2015 9:57 pm
I am a bit stumped by this. Assuming an undamaged column is this even possible?
This is a C18 column, with a pretty non extraordinary HPLC RP protocol using isocratic MeOH/H2O at 55:45 UV monitoring at 230 and 280nm.
I'd throw up more details but really I'm just looking for the basic answer as to whether or not this should even be happening before I raise a ruckus. I'm inclined to question the method and perhaps the fact that we have a peak in the calculated void volume is enough, but test samples keep coming back close to their known concentrations providing an argument that the method and equipment are just fine to proceed with.
The method makes use of different injection volumes for producing the standard curve which I was sure was involved but with a uniform injection volume for the samples we are seeing data that matches (enough) to now rule that out.
Thanks in advance for any thoughts on this.