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- Posts: 44
- Joined: Mon Dec 18, 2017 9:09 pm
Actually, I am planning to do it without any modifier. 2 days ago when i ran the analyte without the modifier i could see both indole 3 carbino (I3C) and the condensation product (DIM) but the ratio of I3C was much higher than DIM and no fronting and tailing of the peak could have seen. So 2 peaks were separated with a good resolution.
However, initially when i tested my analyte in an acidic solvents (20 mM acetic acid) the ratio of I3C and DIM was not that different and in addition the fronting of the first peak (I3C) was merged with the tailing of the second peak (DIM).
So that is the reason i am planning to use a shorter C18 column.