Im injecting blank solvent at the start of the run followed by related substances samples. Its dual wavelength one active at 220nm the other at 240nm, buffer is 20mM potassium phosphate, all standard stuff but I wonder am I doing my prep before the run right. A workmate told me to always blast the column with close to 100%ACN for a bit (10 or 20mins) before you start your run, but by doing that it seems to clog the check valves and that takes up time.
There is so much advice out there about check valves and the best way to activate and prime for gradient buffer runs but none of it is consistent. ANy advice?!