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- Posts: 5
- Joined: Fri Nov 17, 2017 9:36 am
New here, just registered because we're setting up our first HPLC. Recently purcahsed a reconditioned Agilent 1100 with a G1379A degasser, G1312A Binary pump, G1313A Autosampler, G1316A Column oven, and a G1315A DAD.
We know the system passed all diagnostic tests before leaving the place it was reconditioned and serviced, and it's been treated VERY carefully by us ever since.
We ran a leak test from inside Chemstation, which the pump passed no problem. Next, we installed our column (Poroshell 120 EC-C18 2.1x100mm 2.7um), and ran a pressure test.
First test failed, failing to reach >380 bar, and also losing about 7 bar per minute.
So we tightened everything a little (careful not to over-tighten anything), and saw a very slight improvement (but still a fail).
We then moved the blanking nut from the end of the column, to the ALS. This again saw a slight improvement, but again, we're a long way from a pass.
When we moved the blanking nut to the pump outlet, we reached a pressure > 380 Bar, and passed that part of the test, but we're still losing 2.9 bar/min (pass is <2bar/min).
All the documentation seems to suggest that if you've identified the pump as the issue (and I think we've definitely identified it as part of the issue), then run the leak test.
So after this, we ran the leak test again, which the pump passed first time.
Now we're scratching our heads, unsure what exactly is wrong here. Documentation also said it's possibly a damaged/overtightened blanking nut, so we used a brand new one when we moved the nut from the end of the column to the ALS, and used the same new nut at the pump outlet too.
If anyone has any suggestions or input, we'd be super grateful. Thanks in advance! (And sorry if this is in the wrong section. Since it's such a rookie issue I thought it was probably best in Student Projects, but I'll happily repost to Liquid Chromatography if it's better placed there).