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- Posts: 36
- Joined: Mon Jan 02, 2017 1:08 am
As far as I know, the detector should be purged for max. 30 minutes before autozeroing it before purging again.
Thank you.
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
EmpowersBane wrote:
Do you mean as part of a start-up procedure? I use a PDA detector all the time and at the end of my run I flush 90:10 Water:Organic through the system to remove buffer, then 100% Organic for 2 hours before shutting off. Then at the start of the system I light up the lamp for at least 30 minutes before activating run and equilibrate the system with the starting conditions of my mobile phase.
When I get stray peaks or a noisy baseline and I can eliminate the check valves, contamination in my mobile, diluent or standards, then I perform an acid wash of 30% Phosphoric Acid on the entire system and that cleans off any residual drug or rubbish in the flow cell and surrounding connections.
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