PDA detectors?

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

5 posts Page 1 of 1
Hi, can someone please explain the purpose of purging PDA detectors - is it to flush out any residual contaminants/samples from previous run before starting a new run?

As far as I know, the detector should be purged for max. 30 minutes before autozeroing it before purging again.

Thank you.
Do you mean as part of a start-up procedure? I use a PDA detector all the time and at the end of my run I flush 90:10 Water:Organic through the system to remove buffer, then 100% Organic for 2 hours before shutting off. Then at the start of the system I light up the lamp for at least 30 minutes before activating run and equilibrate the system with the starting conditions of my mobile phase.

When I get stray peaks or a noisy baseline and I can eliminate the check valves, contamination in my mobile, diluent or standards, then I perform an acid wash of 30% Phosphoric Acid on the entire system and that cleans off any residual drug or rubbish in the flow cell and surrounding connections.
Perhaps you are confused and writing about the wrong type of detector. We do not purge PDA's. We do purge RID (Refractive Index Detector).
EmpowersBane wrote:
Do you mean as part of a start-up procedure? I use a PDA detector all the time and at the end of my run I flush 90:10 Water:Organic through the system to remove buffer, then 100% Organic for 2 hours before shutting off. Then at the start of the system I light up the lamp for at least 30 minutes before activating run and equilibrate the system with the starting conditions of my mobile phase.

When I get stray peaks or a noisy baseline and I can eliminate the check valves, contamination in my mobile, diluent or standards, then I perform an acid wash of 30% Phosphoric Acid on the entire system and that cleans off any residual drug or rubbish in the flow cell and surrounding connections.

30%.. I think it should be 17,5%.can u explain to me? tks
Please do not wash down the system with acid. What does this have to do with "purging" ?? I think you need to speak with someone at your company who is experienced in chromatography.
5 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry