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- Posts: 1
- Joined: Sun Sep 17, 2017 3:08 am
I'm having a problem in the middle of runs. The column had reached over the maximum limit of standard operation. I tried to wash the column using 100% ACN. At first the pressure is normal which below than 100 bar. But then over time it reached to 350 bar. I'm trying to consider about regenerating my column. But still have no proper procedure. I'm using capillary column C18 (0.5 mm) with silica beads. And mobile phase water,ACN, and acetic acid.