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Determination of benzo[a]pyrene - sample was analyzed by LC with UV detection (ʎ = 254 nm). Benzo[b]chrysene was used as an internal standard - 200 µl (c = 50µg/ml) of standard solution was added to 2 ml of sample. Injection volume of prepared solution sample + standard was 20 µl.
Peak areas:
benzo[a]pyrene A = 3,21 cm2
benzo[b]chrysene A = 1,83 cm2
Molar extinction coefficients (ʎ = 254 nm):
benzo[a]pyrene ɛB(a)P = 39800 mol-1. l . cm-1
benzo[b]chrysene ɛB(b)CH = 24500 mol-1. l . cm-1
Molecular mass:
benzo[a]pyrene Mr(B(a)P) = 252,3
benzo[b]chryseneMr(B(b)CH) = 278,4.
What is the concentration of benzo[a]pyrene in the sample (µg/ml)?
It would help me a lot. Thanks!!