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- Posts: 2
- Joined: Mon Aug 21, 2017 4:44 pm
This is my first time in the forum so I am not sure if this is the right place to ask this question. If not, it would be nice if you could direct me to the proper subforum.
So I am trying to analyze the ascorbic acid content of a sample which has been extracted using a 2.5% Metaphosphoric acid buffer in methanol. For my analysis, I ran my extraction buffer as a blank besides the samples themselves and the standards. The blank yields a peak that has a retention time close to the retention time of my standard. What is puzzling is that the peak from the blank run disappears when the standard (which has been diluted with the buffer) is analyzed, but reappears when I am analyzing the samples themselves. What could be causing the issue? Thank you