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- Posts: 9
- Joined: Tue Oct 11, 2011 7:58 pm
This is my first time posting in this forum and I would very much appreciate somebodys help on this matter.
I am developing an HPLC method for the analysis of phosphonates. This method involves a post column reaction where the phosphonates are converted into ortho-phosphate and detected by UV analysis at 800nm. I have been advised to use an internal standard in my method but I am finding it very difficult to find a compound which absorbs in this region. The internal standard chosen should not be involved in the reaction and be detected independently.
Would anybody have any ideas of what compounds I could maybe use or where I could look for information? Does anyone know if there is an online database or a book which would have the UV spectrum of compounds in it?
Thanks