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- Posts: 3
- Joined: Sun Aug 26, 2012 8:48 pm
Could someone please help me in developing a method on triple quadrupole mass spectrometer. I have a method developed on HPLC-UV and the solvent system used was hexane/Isopropanol +0.1% triethylamine (90:10), and the analytes are basic in nature (Pka around 10). I am planning to develop a method on mass spec. To my knowledge it's difficult to see an ESI spectrum using hexane as a solvent. I did a trial on ESI and APCI by direct injection using the same (as given above) combination of solvents, but I wasn't able to see any peaks. The coulmn is compatible with solvents such as alkanes, alcohols (methanol, ethanol and Isopropanol), and acetonitrile. Could someone please suggest me to develop a method on mass spec. I would be happy to provide more information if needed. Thanks in advance for your reply.
Thanks,
Sravan Mansani.