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HPLC Column Query

Posted: Tue May 21, 2013 1:06 pm
by hello345
Hi guys

I am currently in the first year of my PhD and trying to set up an HPLC method for the analysis of phosphonate scale inhibitors. The method was previously developed a few years ago by a member of staff who is no longer here and although the method works ok I am a bit confused as to why the column used was chosen.

The method uses a Dionex AS11-HC column which is designed for the analysis of anions, however I am analysing scale inhibitors. Does this seem odd to anyone? Why would such a column be chosen?

Any advice would be appreciated :)

Thanks

Re: HPLC Column Query

Posted: Tue May 21, 2013 3:40 pm
by Markus Laeubli, Metrohm
That's not that strange as phosphonates are organic acids based on phosphonic acid See http://en.wikipedia.org/wiki/Phosphonate.

Re: HPLC Column Query

Posted: Tue May 21, 2013 4:15 pm
by sepscientologist
It seems perfectly reasonable to me. That column is good for measuring phosphonates. You are looking for things
that inhibit phosponates so look for the least amount of phosphonates produced.