Help Needed. Waters 510 issues.

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
As the subject says, I'm having issues with my waters 510. No matter how many new lines I put in, they all leak. The strange part is it's leaking between the line and the head piece. Any suggestions?
Sounds like you have a clog in your pump, thus giving you a very high pressure. Take any column offline, then wash with water, then methanol, hexane, IPA, and finally water again. Your final pressure should be very low and the lines should not leak.
If you are using stainless steel nut and ferrule ,for connecting your line , try using PEEK fingertight fitting.
3 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry