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- Posts: 3
- Joined: Wed Sep 20, 2017 2:22 pm
start with a problem:
I can not see sorbic acid on the chromatogram; I explain you
I'm trying to separate sorbic acid from transmuconic acid:
I dissolve the standard of sorbic acid in ethanol and then diluted it in water to inject it onto the hplc; The mobile phase consists of a 1% acetic acid solution (phase A) and methanol (Phase B), the column being a Perkin Elmer "COL - C18 3um 100 x 4.6mm". The standard of transmuconic acid dissolved in ethanol and diluted in water and injected is seen at a retention time of about 5 minutes at 264 nm. Under the same conditions, the peak of sorbic acid does not appear; sorbic acid is very similar to trasmuconic acid , how come you do not see it ??
Can anyone help me understand this arcane?