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- Posts: 15
- Joined: Thu Apr 20, 2017 9:00 pm
I want to get some expert's opinion about how you usually design a calibration curve in a HPLC test. I find limited information about how to do it correctly. AOAC said equal distance design (add different amount of stock solution not weigh standard separately) is better because a true serial design will bias the weight comes from the point with higher concentrations.
For example, which one do you use and why you use it?
A true serial dilution design (made by diluting the last level solution):
1ug/ml, 2ug/ml, 4ug/ml, 8ug/ml, 16ug/ml
An equal distance design (made by adding different amount of stock):
1ug/ml, 5ug/ml, 9ug/ml, 13ug/ml, 17ug/ml
A mixed type of design (made by adding different amount of stock):
1ug/ml, 3ug/ml, 5ug/ml, 10ug/ml, 20ug/ml
I appreciate any inputs. Thank you guys!