PEEK column.

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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I am packing a PEEK column with proteins and chromatography particles. My question is, how effective are they? if anyone has worked with them before, I would really appreciate it any suggestions and tips. Also, if someone can recommend a literature.
IsaacFre92 wrote:
I am packing a PEEK column with proteins and chromatography particles. My question is, how effective are they? if anyone has worked with them before, I would really appreciate it any suggestions and tips. Also, if someone can recommend a literature.


What is your specific question? Is it related to column packing or performance of PEEK tubing for chromatographic purposes.
M. Farooq Wahab
mwahab@ualberta.ca
Hi.
The initial post was not clear.

Perhaps he wanted to make/pack PEEK HPLC column, with some silica beads? He wanted to use 3 or 5 or 10 um? To analyze proteins? In vitamins?

Contact Phenomenex for help ASAP. BTW, did you buy your first HPLC yet?

Alfred
You can also contact Waters, Agilent or Advanced Materials Technology, or ACE, or Hichrom.
Peek column tubing requires a different packing procedure than stainless steel tubing. But most packing methods are kept as a secret.
Good luck.
Gerhard Kratz, Kratz_Gerhard@web.de
Gerhard Kratz wrote:
You can also contact Waters, Agilent or Advanced Materials Technology, or ACE, or Hichrom.
Peek column tubing requires a different packing procedure than stainless steel tubing. But most packing methods are kept as a secret.
Good luck.


As someone who is really interested in demystifying the science of packing, it always saddens me that this science is only throttled because of sheer competition and commercialism. In short, even if know the exact method of xyz company, it fails miserably on another stationary phase chemistry even if the starting particles are the same. In short, you have to develop your own methods based on very simple microscopic tests. If the original poster had followed up, we could have helped him.
M. Farooq Wahab
mwahab@ualberta.ca
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