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- Posts: 50
- Joined: Thu Apr 10, 2014 3:51 am
Thanks.
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Andy Alpert wrote:
Farooq, are you evaluating the 2.1-mm i.d. columns on an HPLC system that's been set up for 4.6-mm i.d. columns? If so, then peaks from the 2.1-mm i.d. column will exhibit severe fronting, due to diffusion into the excessive extracolumn volume. The column itself may be fine. The solution is to increase the flow rate - thereby reducing the time available for diffusion - or to use shorter connections with narrower i.d.'s. Also, use a detector cell with a volume of 1-2 µl instead of the more usual 8-9 µl.
Andy Alpert wrote:
Gerhard is correct.
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